Quantitative fluorescence in situ hybridization on paraffin embedded tissue
M Ricciardi, M Krampera, M Chilosi - Stem Cells and Aging: Methods and …, 2013 - Springer
M Ricciardi, M Krampera, M Chilosi
Stem Cells and Aging: Methods and Protocols, 2013•SpringerQuantitative fluorescence in situ hybridization (Q-FISH) is a complex technique for the
quantitative evaluation of telomere length on cell preparations or on human tissues. The
samples are stained with a fluorescent peptide nucleic acid (PNA) probe against the
telomere oligonucleotides (sequence 5′-TTAGGG-3′). The measure of the telomere
length is carried out using a fluorescence microscope equipped with a sensitive CCD
camera and analyzing the pictures with a computer software that can perform fluorescence …
quantitative evaluation of telomere length on cell preparations or on human tissues. The
samples are stained with a fluorescent peptide nucleic acid (PNA) probe against the
telomere oligonucleotides (sequence 5′-TTAGGG-3′). The measure of the telomere
length is carried out using a fluorescence microscope equipped with a sensitive CCD
camera and analyzing the pictures with a computer software that can perform fluorescence …
Abstract
Quantitative fluorescence in situ hybridization (Q-FISH) is a complex technique for the quantitative evaluation of telomere length on cell preparations or on human tissues. The samples are stained with a fluorescent peptide nucleic acid (PNA) probe against the telomere oligonucleotides (sequence 5′-TTAGGG-3′). The measure of the telomere length is carried out using a fluorescence microscope equipped with a sensitive CCD camera and analyzing the pictures with a computer software that can perform fluorescence intensity measurements. Here, we describe the most used protocols to stain, acquire, and analyze fixed human cells in order to evaluate their telomere length.
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