Triptolide suppresses CD80 and CD86 expressions and IL‐12 production in THP‐1 cells1
J Liu, Q WU, Y FENG, Y YANG, X LI… - Acta Pharmacologica …, 2005 - Wiley Online Library
J Liu, Q WU, Y FENG, Y YANG, X LI, J ZUO
Acta Pharmacologica Sinica, 2005•Wiley Online LibraryAim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium
wilfordii Hook F (TWHF), on the co‐stimulatory molecule expression and interleukin‐12 (IL‐
12) production from THP‐1 cells. Methods: THP‐1 cells were differentiated into macrophage‐
like cells by Me2SO, and then cultured with IFN‐γ (500 kU/L) and lipopolysaccharide
(LPS)(1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on
a FACScan flow cytometer. IL‐12p40, IL‐12p70 were assayed by ELISA. Results: Tripolide …
wilfordii Hook F (TWHF), on the co‐stimulatory molecule expression and interleukin‐12 (IL‐
12) production from THP‐1 cells. Methods: THP‐1 cells were differentiated into macrophage‐
like cells by Me2SO, and then cultured with IFN‐γ (500 kU/L) and lipopolysaccharide
(LPS)(1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on
a FACScan flow cytometer. IL‐12p40, IL‐12p70 were assayed by ELISA. Results: Tripolide …
Abstract
Aim: To investigate the effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F (TWHF), on the co‐stimulatory molecule expression and interleukin‐12 (IL‐12) production from THP‐1 cells.
Methods: THP‐1 cells were differentiated into macrophage‐like cells by Me2SO, and then cultured with IFN‐γ (500 kU/L) and lipopolysaccharide (LPS) (1 mg/L) with or without triptolide. The surface molecule expressions were analyzed on a FACScan flow cytometer. IL‐12p40, IL‐12p70 were assayed by ELISA.
Results: Tripolide suppressed CD80 and CD86 expressions on IFN‐γ (500 kU/L) and LPS (1 mg/L) activated THP‐1 cells at nontoxic dosages of 2.5–0.625 μg/L. Furthermore, the production of IL‐12p40 and IL‐12p70 were also significantly reduced in THP‐1 cells exposed to triptolide.
Conclusion: Triptolide impairs the antigen‐presenting function by inhibiting CD80 and CD86 expressions and decreased IL‐12p40 and IL‐12p70 (bioactive form) productions from the activated THP‐1 cells.
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